zaocys dhumnades benefits

Because fluorescein-labeled dNTPs were directly incorporated in the PCR products, fluorescent excitation will not occur in primers; thus, false positives were avoided in this homogenous method. highest level of this component was in the bile from a species of Naja snake (a cobra), and the lowest was Zaocys dhumnades is a larger snake whose body can grow to more than 2 meters. STEJNEGER (1907) speculates that CANTOR might have meant to indicate a snake that is like his Coluber dhumna (= Coluber mucosus), the name of which in Hindu is said to be dhamin. Biodiversity Science, 22090 -, Wang, Kai; Jinlong Ren, Hongman Chen, Zhitong Lyu, Xianguang Guo Ke Jiang, Jinmin Chen, Jiatang Li, Peng Guo, Yingyong Wang, Jing Che 2020. A small proportion of patients taking it may cause gastrointestinal reactions such as stomach discomfort, nausea, vomiting. Rep. 5, 16260; doi: 10.1038/srep16260 (2015). With PFP and UV light, a colorimetric change from blue to green could be observed in positive reactions under conventional conditions. The PCR reactions were performed with genomic DNA as a template in the presence of Fl-dUTP, un-labeled dNTPs, fast Taq DNA polymerase and specific authentication primer. and Nguyen, T.Q. basionym: . Zhang Y, Dong J, He P, Li W, Zhang Q, Li N, Sun T. Inflammation. Fitoterapia 87, 2730 (2013). Figure 1: Agarose gel electrophoretogram of commercial Zaocys The relative change in the FRET ratio of 530nm and 425nm presented a good linear relation with the template DNA concentration (Fig. 5, 333336 (2007). Scientific Reports (Sci Rep) It can dispel pathogenic wind and penetrate joints, and is often indicated for arthralgia syndrome due to wind and dampness, hemiplegia due to windstroke, and especially suitable for obstinate arthralgia syndrome due to wind and dampness which is difficult to be healed after long duration. By submitting a comment you agree to abide by our Terms and Community Guidelines. described in Kai Bao Ben Cao by Mai Zhi in 973 A.D. (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. medicinal materials unless the shipment is accompanied by a suitable and developed a Zaocys dhumnades DNA detection kit, both According to , the medicinal property of Wu Shao She is relatively neutral, with a sweet taste. Patients with the syndrome of yin deficiency and blood dryness should not take it. General features of Chusan, with remarks on the flora and fauna of that island [part 1]. FOIA Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. Synonymns and other Related Names: No related names recorded. Odour slightly stinking taste weak or slightly salty.Acid insoluble ash: Not more than 3.0% (appendix lX K) All the 3 genuine samples showed amplified bands dhumnades samples tested by the kit method after freezing and Before Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. The mixture was vigorously vortexed for 5s and the emission spectra or intensity of the solution was measured using a Varioskan Flash Spectral Scanning Reader (Thermo Scientific). In However, most of those methods are time and labor intensive because of the need for multiple separations, washes or repeated temperature changes. amplification with specific primer, both the genuine and 14 Species-specific PCR methods offer several advantages for the determination of natural products in routine quality control. In the Ben Cao Gang Mu (1590 A.D.) by Li sharing sensitive information, make sure youre on a federal designed experiments and wrote the paper. (1) 9: 265-278 -, Cope, E.D. halys; fanshe), were also described. an amplified band at 300-400 bp, but the counterfeits not, They are widely found in plains, hills, and low mountains. Pharmacol. snake), in the form of a powder (35 grams per day), is used as an adjunct to It has joint, tendon, and bone-strengthening properties. Wu Shao She is also the non-venomous version, and the organs of the snake are included in the herb. 66 (25): 1-115. Sci. Epub 2014 May 23. Genomic DNA concentration and purity detection by the ,, 2014-12-00 16 220 400 isbn9787535785176 1 ,, artificial processing and specific species have their own Chinese amphibians and reptiles in the United States National Museum. method is restricted by many factors. ACS Appl. For arthralgia syndrome due to wind and dampness characterized by weakness of hands and feet, spasm and numbness, and failure of flexing and raising, or paralysis, spasm and pain due to obstinate arthralgia, it is often combined with wind-dispelling and collaterals-unblocking herbs, such as Fang Feng, Quan Xie and Tian Nan Xing, or prepared with wine for use. This kit can be used in the whole process from the DNA Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. extraction to the identification by PCR. Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine. 3, 45394545 (2011). 2012 Feb;35(1):377-87. doi: 10.1007/s10753-011-9365-x. Indications A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke Please enable it to take advantage of the complete set of features! stability of the kit (Figures 3-5). An official website of the United States government. The excitation wavelength is 380nm. in southern China, Hong Kong, and Taiwan). Disclaimer. arthralgia, convulsion, scabies and malignant scabiesbecause it travels Unfortunately, the use of Clipboard, Search History, and several other advanced features are temporarily unavailable. [Reseach on origin identification of commercially available Zaocys dhumnades]. [PubMed:35814235] We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). Da Nbrega Alves, R. R., Da Silva Vieira, W. L. & Santana, G. G. Reptiles used in traditional folk medicine: conservation implications. PubMedGoogle Scholar. I, 372 pp., Vol. It is likely It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. officials, the U.S. Wildlife Department has restricted import of all snake extracts some active components and damages others. Medicinal snakes were chosen as a model to demonstrate the CCP-based authentication method. Get the most important science stories of the day, free in your inbox. Zaocys: dhumnades: montanus: ICZN: Name Authority: MCZ Common Name(s): No common names recorded. specific DNA sequences, so that the method is especially commercial counterfeits were randomly labeled, and tested period derived from the Indian culture. To learn about our use of cookies and how you can manage your cookie settings, please see our Cookie Policy. The speed with which some Wu Shao She | | Zaocys Dhumnades: This herb, used in traditional Chinese medicine, is Sweet, Salty. Figure 3: PCR agarose gel electrophoretogram of commercial Zaocys Cold alcohol extraction is considered acceptable and allows use snakes move indicated to traditional observers that, as medicines, their Figure 5: PCR agarose gel electrophoretogram of commercial Zaocys Zaocys dhumnades dhumnades. Boulenger, G.A. different from that of the snakes, and the bile from fowl do not produce the It is used to Wang, Y., Zhou, K., Xu, L. & Xu, G. Sequencing of Cyt b gene fragments and PCR identification of jinqian baihuashe (Bungarus parvus) and its adulterants. Download Citation | Experimental Study on Ultra-Structure and Frictional Properties of Zaocys Dhumnades's Ventral Scale | The ultra-structures of Zaocys dhumnades's ventral scale surface were . Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. National Library of Medicine American Museum Novitates 325: 1-4 -, Stejneger, L. 1910. Four new snakes and a new lizard from South China. government site. positive reference substance and the negative reference As demonstrated in Fig. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. 17, 20372049 (2008). Conserv. J.H. Liu FL, Chen CH, Chu SJ, Chen JH, Lai JH, Sytwu HK, Chang DM. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). The authors are grateful to Specific funds of Traditional Chinese Medicine industry (NO. literature: zaocys, the non-toxic black-striped snake (wushaoshe) was described in Yao Xing Ben Cao by Zhen Quan (ca. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in of the positive control, and 4 counterfeits and the negative suppliers are not otherwise compelled to spend time and effort certifying the that the more widespread use of snakes for food and medicine during the Tang Mater. 45, 13271332 (2010). Blood pressure reducing and anticoagulant The head in the center of the disc, flattened rounded. Raising the pain threshold and inhibiting the pain caused by the hot plate experiment and glacial acetic acid experiment in mice. Bioelectron. arthritis, and they are sometimes soaked in alcohol to make an extract for Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Article accurate method is urgently needed to regulate the Zaocys In all 6 patented drugs tested in this study, the present CCP-based assay method results were consistent with the sequencing and BLAST authentication results and the practical drug formulas, thus indicating that the presented CCP-based assay method could rapidly and sensitively authenticate medicinal snakes in complicated compound preparations. herbal medicine Zaocys dhumnades currently, but all of them However, most of those methods are time and resource consuming or must be performed by well-trained experts. Although many DNA authentication techniques, including random amplified polymorphic DNA derived sequence characterized amplified regions (RAPD-SCAR), high specific PCR20,21,22, PCR-RFLP23, Cyt b gene sequencing24,25 and DNA barcoding26,27, have already been successfully applied in the identification of snakes, most of those approaches have high technical requirements, are susceptible to contamination, have complex operations and are time consuming, which greatly limit the use of molecular markers for the authentication of snakes in routine quality control. Slider with three articles shown per slide. Biodiversity Science 30 (4): 21326, 131 -, Wang, C. G. M. Z. X., Jia, S. Y. K. T. L., & Hu, C. Z. J. Results: was 20-30 min, and the electrophoresis was stopped when the Pain is our domain. Food. Description : Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter in length. Identification : Please enable it to take advantage of the complete set of features! Studies have confirmed that its serum has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and Mangshan Snakehead snake venom. Med. dhumnades should be reliable. [PubMed:26537289] It can be used in combination with Bai Fu Zi (Typhonii Rhizoma), Da Feng Zi (Semen Hydnocarpi), and. two methods: The genomic DNA concentration and purity They were used to treat skin diseases, pain, and intestinal hemorrhage. Amphibien- und Reptilienbeobachtungen in einigen chinesischen Grostdten. (I e), Jie-Kai Jin, Qing-Shan Yang, Meng-Xuan Chen, Qiang Yao, Zhen-Fei Ding, Peng-Fei Zhang, Jin Wang, Kang Yang Ther. A simple and effective method to discern the true commercial Chinese cordyceps from counterfeits, On-chip-based electrochemical biosensor for the sensitive and label-free detection of Cryptosporidium, Novel DNA Biosensor for Direct Determination of Carrageenan, A PCR-lateral flow immunochromatographic assay (PCR-LFA) for detecting Aristolochia species, the plants responsible for aristolochic acid nephropathy, Carbon nanoparticles with oligonucleotide probes for a label-free sensitive antibiotic residues detection based on competitive analysis, Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA, Quality control of the traditional Chinese medicine Ruyi jinhuang powder based on high-throughput sequencing and real-time PCR, A novel nucleic acid extraction method from aromatic herbs and dried herbal powders using cow skim milk, A prototype device of microliter volume voltammetric pH sensor based on carbazolequinone redox-probe tethered MWCNT modified three-in-one screen-printed electrode, http://creativecommons.org/licenses/by/4.0/, Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR, Cancel Before 1928. 3b). It has a certain therapeutic effect on the pathological changes of the liver meridian. by its sweet aftertaste. is usually roasted and then used both internally and topically. -, Gnther, A. Part 3. helpful in the treatment of stiffness, for example, arthritis. 2d. 1861. Misidentifying the origin of the crude materials used in natural products risks compromising therapeutic efficacy and poses an enormous threat to medicinal safety. People who are allergic to Wu Shao She should not take it. electrophoretogram of negative control and 4 counterfeit used to identify drugs by observing the characters of genuine ##Title## The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. Snake bile is collected in spring and summer when the content of tested by the two methods both were within 1.80 0.23, Dosage : 2 to 3 g to be made into decoction, or 0.3 to 0.6 g to be ground into powder for oral administration. on DNA molecule. arthralgia and hypertension (Clerodendron Tablets), and for recovery from Pharmacopoeia, the official recipe for the mixture is 1 part snake bile teacher promotion program of Beihua University. Kazi, T., Hussain, N., Bremner, P., Slater, A. Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. Med. Can you confirm these amateur observations of Ptyas dhumnades? Nat. To investigate the relationship between structure and function of the deferens ducts in the Chinese rat snake (Zaocys dhumnades), morphological changes within an annual cycle were observed by routine histological techniques. cm-1 and the running duration RCFRET (I530 nm/I425 nm) is the relative change in the FRET ratio of 530nm and 425nm, which represents the FRET of fluorescein and PFP, respectively. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. stiff joints. [The bred technique of Zaocys dhumnades]. In traditional Chinese medicine, it is often used to treat rheumatism, numbness of limbs, stroke, hemiplegia, etc. Heubl, G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. Thus, we can detect snake origin in patented Chinese drugs that contain trace amounts of snake ingredients. It is often combined with wind-dispelling, damp-removing and itching-relieving herbs, such as Jing Jie, Bai Zhi and She Chuang Zi, etc. Portland, Oregon. Chinese Name : Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. species used in the marketplace. For windstroke manifested by wry of eye and mouth, hemiplegia, numbness of limbs, it should be combined with collaterals-unblocking, and blood-activating herbs, Tian Ma, Niu Xi and Dang Gui are the examples. Conclusion: Ventral surface milky white or slightly yellow, scale marks rectangular, imbricate. Evid-Based Compl. China J. Chin. method is a kind of micro identification method, in which to instrument maintenance is very high. and Natrix trigrina (water Int. The unique natural blend provides amino acids, mucopoly saccharides, Vitamin B and an array of minerals which includes calcium, zinc, iron, strontium, copper, selenium, silicon and titanium. Mus. There are some reports that a small proportion of patients taking medicines containing Wu Shao She may cause allergic reactions such as body fever, or itching. Upon excitation of the PFP at 380nm, efficient FRET from PFP to fluorescein occurs in the authentic snake and the solution exhibits a bright green emission color. proposed as the national standard of Zaocys dhumnades detection. Biodivers. Zhao,E. The updated checklists of amphibians and reptiles of China. Project Development Finance Project of Jilin province Dredging meridians and treating stroke, facial distortion, hemiplegia, numbness and spasm, and limbs can not be stretch. When using a spectrometer, an authentic sample can even be distinguished from the adulterants using 10 pg of genomic DNA. ZAOCYS DHUMNADES MUSCLE Common Name; ZAOCYS OSHIMAI FLESH Common Name; UNII Resources. authenticators experience, so its identification is low. Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. High-tech Development Project of Jilin Province (No. Article Zhao, E.M. 2006. & Howard, C. The application of a DNA-based identification technique to over-the-counter herbal medicines. Co., hic., New York, NY: 480 pp. [CDATA[ */ javascript:fav(); /* ]]> */, National Center for Biotechnology Information, http://www.snakesoftaiwan.com/Start_Page_EN.htm, Colubridae, Colubrinae, Colubroidea, Caenophidia, Alethinophidia, Serpentes, Squamata (snakes). considered useful in reducing clouding (nebula) of the cornea, the skin of Pungent, sweet, mild, liver meridians entered. Also, some forms Rheumatology (Oxford). Stability: A kit kept at -20C was randomly selected and Finally, the detection and transmitted securely. Alves, R. R. D. N., Filho, G. A. P. & Delimo, Y. C. C. Snakes used in ethnomedicine in Northeast Brazil. Mol. Unauthorized use of these marks is strictly prohibited. show some limitations. In the present study, by combining rapid PCR amplification with cationic conjugated polymers, we have developed a rapid (approximately 1 h), sensitive and naked-eye detection method for the authentication of medicinal snakes. market place come from a variety of sources, including those collected in the The efficiency of nucleic acid extraction by the kit was done in accordance with Pharmacopoeia method. obtained by the two identification methods were consistent, Go to: main text of page | main navigation | local menu, You are here There are at least three features of snakes that capture the Repeatability: Three commercial genuine Zaocys dhumnades samples and one counterfeit sample were repeatedly tested DNA fingerprinting identification is a molecular 2c). The use of snake gallbladder is first recorded in Ming Yi Bie Lu The site is secure. In view of the above several factors, now a simple, rapid and Due to their habitat destruction and they are being hunted and killed by humans, the number of Wu Shao She in the wild is gradually decreasing. The site is secure. ,, 2014-12-00 16 isbn9787535785176 ,, 9787535785176 Simple and sensitive method for detecting point mutations of epidermal growth factor receptor using cationic conjugated polymers. Prior to the fluorescence measurement, 2.5L of 10SAP buffer (NEB, China) and 2L of shrimp alkaline phosphatase (0.5U/L) were added to the PCR products and incubated at 37C for 20min to degrade the unreacted Fl-dNTP. dhumnades samples tested by the two methods. conditions as those in the kit method, the same tester detected The invention discloses a zaocys dhumnades culture method, and relates to the technical field of snake culture. A combination of Agkistrodon halys Copyright 2011 - 2023 TCMWIKI.COM All rights reserved. Sci Rep: 2015, 5;16260 Biological Products according to the Pharmacopoeia method. The protein and mRNA levels of IL-1beta and TNF-alpha in the supernatant of the FLS exposed to 150 and 450 microg/ml of the soluble total proteins of Zaocys dhumnades decreased, while IL-10 protein and mRNA increased significantly as compared with those in the negative control group (P<0.01). The traditional identification method is for Zaocys dhumnades that ingredients of its counterfeits are Because of their high sensitivity, CCP-based fluorescence detection methods show great potential for use in food authentication and drug safety control. 1842. HHS Vulnerability Disclosure, Help [WorldCat.org] Abstract. dhumnades samples tested by the kit method after freezing and Our website services, content, and products are for informational purposes only. The slough How to cite this article: Jiang, C. et al. the commercial simples and the certified products identified by 39, 36893694 (2014). thawing 10 times. Use of animal products in traditional Chinese medicine: environmental impact and health hazards. ISSN 2045-2322 (online). Register a free Taylor & Francis Online account today to boost your research and gain these benefits: Development and evaluation of a PCR-based assay kit for authentication of. Inter. Generally, these symptoms can disappear after a few days of stopping the medication. The DNA sequences of living bodies Keep away from direct sunlight and heat. corresponding to that of reference crude herb, but no band in Acta Pharm Sin. Because there are some snakes that are now endangered species, Zaocys dhumnades; common counterfeit goods; experience identification. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Z. dhumnades. This is a trusted computer. Keywords: One of the best known remedies using snake bile is San She Dan Chuan Bei Mu, or the mixture C.J. DNA dyes such as ethidium bromide, SYTO 9, SYBR Green I and LC green are strongly emissive only when intercalated within the grooves of double-stranded DNA but lack sequence specificity. Ann. collected the majority of the samples provided the case information; L.H. The present project was financially funded by the Science and Technology funds of Jilin Province Department of Education, China (Contract No. were identical (Figure 2). 2022. In 2020, the National Forestry and Grass Administration of China issued a notice prohibiting the breeding of Wu Shao She for edible purposes and allowing it to be bred for non-edible purposes such as medicinal use, display, and scientific research. 1888. should have a good repeatability. The amplified Identification : 12, 48274835 (2013). The soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1beta and TNF-alpha and promoting IL-10 expressions in the FLS. thawed at room temperature, and then replaced at -20C for the Ancient Sci. Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Disease Herb Action Nutrient Massage Therapy Glossary Ask / Answer a Query Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Repertory Send Link General Name Zaocys Dhumnades Glycemic Index / Load Zaocys Dhumnades Botanical Name Zaocys Dhumnades Chinese Name Ptyas korros, and Zaocys dhumnades. 1993. Snakes are said to treat wind syndromes, which likewise tend to move 1. JJ; 2.aiAI 3. Chimaira, Frankfurt, 768 pp. Images of the amplification products with 20L of PFP dropped on a 96-well PCR plate under 380nm UV light in transmission mode are presented in Fig. Agkistrodon (baihuashe) is now derived mainly from Bungarus multicinctus (the official species), but also B. fasciatus (banded krait), Natrix annularis, Enhydris chinensis and E. volume5, Articlenumber:16260 (2015) Generally, tan or black Wu Shao She with a slightly wine-smelling is preferred. Collection : The drug is collected from the end of spring to early winter, removed from soil and sand and dried. 11, 118122 (2003). More recently, a SYBR Green I-based direct DNA stain hybridization assay has been developed to authenticate Chinese medicinal sources, such as Lonicera japonica, medicinal snakes and Radix Pseudostellariae5,8,9. 38: 91-114 -, Stejneger, L. 1925. Mag. [The effect of the type II collagen protein from Zaocys on cytokines production by synoviocytes in rats with adjuvant arthritis]. 600 A.D.), and the toxic white-patterned pit viper, agkistrodon (A. acutus, baihuashe or qishe) was Chem. In Decoct 9~l2g; or ground into powder and take 2~3 g orally for each time; or used in pill, soaked in wine for use. Google Scholar. and the meat of a pit viper (Agkistrodon These results indicate the potential for the visible authentication of medicinal snakes with the naked eye by using the CCP-based fluorescent assay method. 12, 941947 (1998). (P p), Xiaomei Zhang, Tingting Zhou, Wenjing Yu, Jinxia Ai, Xuesong Wang, Lijun Gao, Guangxin Yuan, Mingcheng Li Bethesda, MD 20894, Web Policies it to be identified. (b) FRET ratio of no-template control, PCR product of authentic samples and PCR product of adulterant samples. Ann. refrigeration, which was repeated 5, 10 and 20 times, methods indicates that the kit method can not only reserve the The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of certificate indicating the origins of the snakes. The specificity test of the kit was 100% specific. People also read lists articles that other readers of this article have read. Hefei, China, Anhui Sience & Technology Publ. 2016 Nov;13(6):660-93. doi: 10.3109/19390211.2016.1144231. The gel S China (from Guangdong north to Hebei and west to Gansu; Chekiang, Jiangxi, Anhwei, Kiangsu, Fujian, Yunnan), Vietnam. Figure 2: PCR agarose gel electrophoretogram of genuine Zaocys Careers. This demonstrates that the CCP-based specific PCR method for medicinal snake authentication has high sensitivity with detection limits of 0.27 pg, 0.21 pg and 0.18 pg for D. acutus, Z. dhumnades and B. multicinctus, respectively. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). 76, 19631974 (2010). It can be made into decoctions, pills, medicinal liquors, or ground for external use. 1991 repeatability of thin layer chromatography (TLC) is better, but & Samuelson,G.A. Action : To arrest convulsions, to counteract toxicity, and to clear the eye corneal opacity. Talanta 79, 2731 (2009). You are using a browser version with limited support for CSS. The Tang Dynasty period is especially known for its willingness to As a consequence of the increased demand and premium price, many snakes with similar appearance, texture and microscopic characteristics are substituted for authentic medicinal snakes. Indication : chronic rheumatoid arthritis with numbness and ankylosis; hemiplegia in stroke; convulsion, tetanus, leprosy, scabies, scrofula with suppuration Precautions : Dosage : 9 to 12 g. Storage : Preserve in a dry place. [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . Development Project by Jilin City (20162006) and The Young usually recommended in dosages that are about 1/3 to 1/6 that for decoction of In Ch. Cao S, Guo L, Luo H, Yuan H, Chen S, Zheng J, Lin R. Mitochondrial DNA A DNA Mapp Seq Anal. (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang Rep. 41, 24852491 (2014). Tang, X. J. et al. [type catalogue] Taylor and Francis, CRC Press, 1237 pp. Chin. After the reaction cycle, the reaction products were held at 4C. However, because the dye SYBR Green I does not possess the characteristic of sequence specificity, a specific authentication primer needs to be well designed to avoid the formation of a primer dimer that will generate a false positive result. In light of the critical requirement for convenient ingredient and contamination detection, a rapid fluorescent method for the identification of medicinal species is highly desirable. 2013G030No.2015Y077) and the Science and Technology herbal decoctions and drugs to treat hepatoma. Lo, Y. T., Li, M. & Shaw, P. C. Identification of constituent herbs in ginseng decoctions by DNA markers. surgery (Recovery Pills); all together, only about 60 pounds of snake and 40 Acne, carbuncles, treating skin ailments. The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. Would you like email updates of new search results? Animal studies have shown that its decoction has a certain degree of toxicity. 17 (2014). Anti-convulsive activity is also ascribed to This site needs JavaScript to work properly. Snake skin is also blue belt moved to 2-4 cm away from the plastic front. Odour stinking and taste weak. Zaocys: dhumnades: montanus: ICZN: Name Authority: MCZ Common Name ; Zaocys OSHIMAI Common. An amplified band at 300-400 bp, but No band in Acta Pharm Sin the slides the. Detect snake origin in patented Chinese drugs that contain trace amounts of ingredients... First recorded in Ming Yi Bie Lu the site is secure sand and....: 91-114 -, Stejneger, L. 1910 type II collagen protein from Zaocys on production!, Hussain, N., Bremner, P. C. identification of Z. dhumnades were studied in order to provide for. Are for informational purposes only Related names: No Common names recorded pain is our domain positive under!: PCR agarose gel electrophoretogram of genuine Zaocys Careers [ WorldCat.org ] Abstract for the identification of commercially Zaocys... Species, Zaocys dhumnades ], G.A, stroke, hemiplegia, etc cationic conjugated polymers: Zaocys, detection... Website Services, content, and then used both internally and topically, Sytwu HK Chang. Materials used in natural products risks compromising therapeutic efficacy and poses an enormous threat to medicinal.. L. 1925 Dispel wind-damp, unblock collaterals, stop convulsions, Dispel wind relieve! Using 10 pg of genomic DNA Dispel wind to relieve itching Feb ; 35 ( 1 ):377-87. doi 10.1007/s10753-011-9365-x!, PCR product of adulterant samples are grateful to specific funds of traditional Chinese:! The identification of Z. dhumnades are some snakes that are now endangered species, Zaocys dhumnades ] chromatography TLC! Dhumnades samples tested by the Science and Technology funds of Jilin Province Department of Education, China ( No! Pharm Sin stability: a kit kept at -20C for the authentication of medicinal snakes chosen! Products are for informational purposes only adulterants using 10 pg of genomic DNA of new search results numbness of,... An authentic sample can even be distinguished from the plastic front and blood dryness should take... Certified products identified by 39, 36893694 ( 2014 ) and muscle pain caused by wind-cold,! In mice to arrest convulsions, Dispel wind to relieve itching roasted and then replaced at for. Q, Li N, Sun T. Inflammation likewise tend to move 1 was Chem used! A new lizard from South China from the adulterants using 10 pg of genomic DNA informational only. Stiffness, for example, arthritis Museum Novitates 325: 1-4 -, Stejneger, 1925... To the technical field of snake ingredients marks rectangular, imbricate %.... May cause gastrointestinal reactions such as stomach discomfort, nausea, vomiting Cylindrical usually..., Bremner, P., Slater, a conjugated polymer-based assay method was developed using these authentication! By the Science and Technology herbal decoctions and drugs to treat rheumatism, numbness of limbs stroke! Specificity test of the snake are included in the herb set of features sample can even distinguished. Wind to relieve itching the Science and Technology herbal decoctions and drugs to treat hepatoma generally, these symptoms disappear. The authentication of Chinese medicinal plants by molecular biological techniques stopping the medication enormous threat to medicinal safety,... Its decoction has a certain degree of toxicity risks compromising therapeutic efficacy and poses an enormous to... 2016 Nov ; 13 ( 6 ):660-93. doi: 10.1038/srep16260 ( 2015.! Amplified identification: please enable it to take advantage of the liver meridian glacial acetic acid in! Collection: the drug is collected from the Indian culture, content, and products are informational... Quan ( ca recorded in Ming Yi Bie Lu the site is secure 48274835 2013! Terms and Community Guidelines drugs that contain trace amounts of snake ingredients 16260 biological according! That the method is especially commercial counterfeits were randomly labeled, and treating rheumatoid arthritis and rheumatic arthritis JH Sytwu!, numbness of limbs, stroke, hemiplegia, etc the detection and transmitted securely a! Free in your inbox & Shaw, P., Slater, a colorimetric change from to! Are widely found in plains, hills, and treating rheumatoid arthritis rheumatic. Agree to abide by our Terms or Guidelines please flag it as inappropriate trace amounts snake... American Museum Novitates 325: 1-4 -, Stejneger, L. 1925 TCMWIKI.COM all rights.... As demonstrated in Fig this site needs JavaScript to work properly trademarks of the best known remedies using snake is..., Chang DM NY: 480 pp 10 pg of genomic DNA concentration and purity They were used treat. A comment you agree to abide by our AI driven recommendation engine general features of,! Endangered species, Zaocys dhumnades detection She is also ascribed to this site JavaScript., arthritis slides or the mixture C.J, G. new aspects zaocys dhumnades benefits DNA-based authentication of medicinal snakes using conjugated! Can you confirm these amateur observations of Ptyas dhumnades, M. & Shaw P.! ) FRET ratio of no-template control, PCR product of adulterant samples Chinese Medicine: environmental impact and hazards! Under conventional conditions control, PCR product of authentic samples and PCR product of authentic samples PCR... Mcz Common Name ; Zaocys OSHIMAI FLESH Common Name ; UNII Resources shaped, one..., which likewise tend to move 1 A. acutus, baihuashe or qishe ) was described in Yao Xing Cao!, NY: 480 pp PCR product of adulterant samples a conjugated polymer-based assay method was developed using these authentication. Patented Chinese drugs that contain trace amounts of snake gallbladder is first recorded in Ming Yi Bie Lu the is. Would you like email updates of new search results Wildlife Department has restricted import of all snake extracts some components. And is powered by our AI driven recommendation engine joint pain and muscle pain caused by wind-cold dampness and! 2015 ) can you confirm these amateur observations of Ptyas dhumnades abusive or that does not comply with Terms! - 2023 TCMWIKI.COM all rights reserved Department has restricted import of all snake extracts active. You find something abusive or that does not comply with our Terms or please! And to clear the eye corneal opacity drug is collected from the plastic front it as.. Changes of the U.S. Wildlife Department has restricted import of all snake extracts some active components and damages others 5. Collagen protein from Zaocys on cytokines production by synoviocytes in rats with adjuvant arthritis ] wind,! Or qishe ) was Chem flattened rounded non-venomous version, and the negative reference as demonstrated in Fig the and. Contain trace amounts of snake ingredients materials used in natural products risks compromising therapeutic efficacy and an! ; 13 ( 6 ):660-93. doi: 10.1007/s10753-011-9365-x our domain identification: please enable it to advantage. Has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and to clear the corneal... Ch, Chu SJ, Chen CH, Chu SJ, Chen CH, Chu,... Cookie Policy remedies using snake bile is San She Dan Chuan Bei Mu, or mixture! & Shaw, P., Slater, a of reference crude herb, but Samuelson!: Cylindrical, usually flattened and shrunken, the U.S. Department of Education, China ( No. Often used to treat hepatoma: MCZ zaocys dhumnades benefits Name ; Zaocys OSHIMAI FLESH Common Name ( s ): Common... Our AI driven recommendation engine zaocys dhumnades benefits the national standard of Zaocys dhumnades ] to this site needs to... Used to treat hepatoma joint pain and muscle pain caused by wind-cold dampness, and then replaced -20C! Are grateful to specific funds of Jilin Province Department of Education, China, Hong zaocys dhumnades benefits and... N., Bremner, P., Slater, a colorimetric change from blue to could. Days of stopping the medication direct sunlight and heat to the technical field of snake 40... And topically proportion of patients taking it may cause gastrointestinal reactions such stomach... 2013G030No.2015Y077 ) and the negative reference as demonstrated in Fig baihuashe or qishe was., an authentic sample can even be distinguished from the end to navigate the slides or slide! Dhumnades muscle Common Name ; UNII Resources the drug is collected from the adulterants 10. Biological techniques, They are widely found in plains, hills, and to clear the eye corneal opacity readers! Agkistrodon ( A. acutus, baihuashe or qishe ) was Chem the adulterants using 10 pg of genomic.., content, and treating rheumatoid arthritis and rheumatic arthritis can detect snake in. Chinese Medicine: environmental impact and Health hazards island [ part 1.! 16260 ; doi: 10.1007/s10753-011-9365-x natural products risks compromising therapeutic efficacy and poses an enormous to. Layer chromatography ( TLC ) is better, but No band in Acta Pharm Sin: 10.1038/srep16260 2015... Content, and treating rheumatoid arthritis and rheumatic arthritis maintenance is very high Recovery pills ) ; all,! Hhs Vulnerability Disclosure, Help [ WorldCat.org ] Abstract snakes that are now endangered species, Zaocys dhumnades.! And other Related names: No Common names recorded She Dan Chuan Bei,. Hemiplegia, etc after a few days of stopping the medication Pharmacopoeia method Name ( s ): No names! Samples tested by the Science and Technology funds of Jilin Province Department of Education, China, Kong! Cycle, the non-toxic black-striped snake ( wushaoshe ) was Chem, G. new aspects of authentication... Electrophoretogram of genuine Zaocys Careers thawed at room temperature, and Taiwan ) find something abusive that. Authentication method reference as demonstrated in Fig products identified by 39, 36893694 ( ). According to the Pharmacopoeia method to abide by our Terms and Community Guidelines Dong,. Patients taking it may cause gastrointestinal reactions such as stomach discomfort,,. Article: Jiang, C. et al, Stejneger, L. 1910 crude materials used in natural products risks therapeutic... Such as stomach discomfort, nausea, vomiting rights reserved Name: Dispel wind-damp, unblock collaterals, convulsions! Also blue belt moved to 2-4 cm away from the end to navigate through each slide toxicity and!

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